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pbluescript sk ii (+) (pbs-skii) backbone plasmid  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript sk ii (+) (pbs-skii) backbone plasmid
    Pbluescript Sk Ii (+) (Pbs Skii) Backbone Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk+(pbs)+plasmid/pm30760763-169-12-19
    Average 90 stars, based on 1 article reviews
    pbluescript sk ii (+) (pbs-skii) backbone plasmid - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryonic development
    Article Snippet: Samples were sectioned into 20 μm‐thick slices using a cryotome (Leica CM 1850; Leica biosystems, Nussloch, Germany). .. (pBluescript [pBS]‐SK, Stratagene, La Jolla, CA, USA) plasmid, which contains full length chicken SHH was used to transcribe sense and anti‐sense cRNA labelled by digoxigenin according to the manufacturer's instructions (Roche Diagnostics GmbH, Mannheim, Germany). ..

    Article Title: A Tale of Two Transitions: The Unfolding Mechanism of the prfA RNA Thermosensor.
    Article Snippet: RNA thermosensors (RNATs), found in the 5′ untranslated region (UTR) of some bacterial messenger RNAs (mRNAs), control the translation of the downstream gene in a temperature-dependent manner.. In Listeria monocytogenes, the expression of a key transcription factor, PrfA, is mediated by an RNAT in its 5′ UTR.. PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the expression of many virulence factors.

    Article Title: Identification of Genes Associated with the Long-Gut-Persistence Phenotype of the Probiotic Lactobacillus johnsonii Strain NCC533 Using a Combination of Genomics and Transcriptome Analysis
    Article Snippet: .. The two amplicons were digested with the corresponding enzymes and ligated together in the cloning vector pBluescript pBS SK+ (Stratagene) digested with EcoRI plus ClaI, the ligated construct was used to transform E. coli XL1-Blue, and chloramphenicol-resistant clones were selected. ..

    other:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: The full-length PfMSH2-1 and PfMSH2-2 genes cloned into the pBlueScript SK+ vector (pBS-SK+; Stratagene) were transformed in separate experiments into the CC108 strain.

    Article Title: Highly Efficient Transgenesis in Ferrets Using CRISPR/Cas9-Mediated Homology-Independent Insertion at the ROSA26 Locus.
    Article Snippet: To generate the transgene donor plasmid, the transgene cassette was cloned into pBlueScript SK II (+) (pBS-SKII) backbone plasmid (Agilent Technologies, La Jolla, CA) by multiple subcloning steps.

    Article Title: Lim15/Dmc1 enhances DNA topoisomerase II catenation activity independent of sequence homology.
    Article Snippet: We reported previously that Coprinus cinereus Lim15/Dmc1 (CcLim15), a meiosis-specific recA-like protein, could specifically activate C. cinereus DNA topoisomerase II (CcTopII).. In particular, it enhanced the catenation activity of CcTopII in vitro at the meiotic prophase stage (Iwabata K, Koshiyama A, Yamaguchi T, Sugawara H, Hamada NF, Namekawa HS, Ishii S, Ishizaki T, Chiku H, Nara T, Sakaguchi K, Nucleic Acids Res, 33:5809–5818, 2005).. In this study, the interaction between CcTopII and CcLim15, especially during catenation, was investigated in detail using atomic force microscopy.

    Article Title: Identification of novel factors that block programmed cell death or apoptosis by targeting JNK
    Article Snippet: To prime in vitro reactions, cDNAs were cloned into the pBluescript (pBS) SK−plasmid (Stratagene).

    Article Title: Method of producing lipid
    Article Snippet: Furthermore, the vector may also be a vector such as a plasmid capable of self-proliferation and self-replication outside the chromosome, or may also be a vector which is incorporated into the chromosome.

    Cloning:

    Article Title: A Tale of Two Transitions: The Unfolding Mechanism of the prfA RNA Thermosensor.
    Article Snippet: RNA thermosensors (RNATs), found in the 5′ untranslated region (UTR) of some bacterial messenger RNAs (mRNAs), control the translation of the downstream gene in a temperature-dependent manner.. In Listeria monocytogenes, the expression of a key transcription factor, PrfA, is mediated by an RNAT in its 5′ UTR.. PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the expression of many virulence factors.

    Article Title: Identification of Genes Associated with the Long-Gut-Persistence Phenotype of the Probiotic Lactobacillus johnsonii Strain NCC533 Using a Combination of Genomics and Transcriptome Analysis
    Article Snippet: .. The two amplicons were digested with the corresponding enzymes and ligated together in the cloning vector pBluescript pBS SK+ (Stratagene) digested with EcoRI plus ClaI, the ligated construct was used to transform E. coli XL1-Blue, and chloramphenicol-resistant clones were selected. ..

    Construct:

    Article Title: Identification of Genes Associated with the Long-Gut-Persistence Phenotype of the Probiotic Lactobacillus johnsonii Strain NCC533 Using a Combination of Genomics and Transcriptome Analysis
    Article Snippet: .. The two amplicons were digested with the corresponding enzymes and ligated together in the cloning vector pBluescript pBS SK+ (Stratagene) digested with EcoRI plus ClaI, the ligated construct was used to transform E. coli XL1-Blue, and chloramphenicol-resistant clones were selected. ..

    Clone Assay:

    Article Title: Identification of Genes Associated with the Long-Gut-Persistence Phenotype of the Probiotic Lactobacillus johnsonii Strain NCC533 Using a Combination of Genomics and Transcriptome Analysis
    Article Snippet: .. The two amplicons were digested with the corresponding enzymes and ligated together in the cloning vector pBluescript pBS SK+ (Stratagene) digested with EcoRI plus ClaI, the ligated construct was used to transform E. coli XL1-Blue, and chloramphenicol-resistant clones were selected. ..



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    (A) A <t>pBluescript</t> II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.
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    Agilent technologies pbluescript sk+(pbs) plasmid
    (A) A <t>pBluescript</t> II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.
    Pbluescript Sk+(Pbs) Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk+(pbs)+plasmid/pm10698494-202-14-19
    Average 90 stars, based on 1 article reviews
    pbluescript sk+(pbs) plasmid - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    (A) A pBluescript II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.

    Journal: PLOS ONE

    Article Title: DAPE cloning with modified primers for producing designated lengths of 3’ single-stranded ends in PCR products

    doi: 10.1371/journal.pone.0318015

    Figure Lengend Snippet: (A) A pBluescript II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.

    Article Snippet: 10 ng of pBluescript II SK+ was used as the PCR template. pX330-puro was a gift from Sandra Martha Gomes Dias (Addgene plasmid # 110403; http://n2t.net/addgene:110403 ; RRID:Addgene 110403).

    Techniques: Plasmid Preparation, Modification, Transformation Assay, Purification, Control